A novel preparation method of smart responsive glass sphere based composite carriers was reported in this study. Firstly, random block copolymers were synthesized by free radical polymerization of N⁃isopropylacrylamide, hydroxypropyl methacrylate and 3⁃trimethoxysilypropyl methacrylate. Then, the copolymer solution was uniformly sprayed on the surfaces of glass spheres by means of a self⁃made bottom spray fluidized bed reactor, and the bonding between copolymers and glass spheres was fabricated by thermal annealing, so as to form the copolymer/glass sphere composite carriers. The coating effects of the smart copolymers on the surfaces of glass spheres were investigated, including characteristic functional groups, surface microstructure, biocompatibility and thermosensibility. The results show that the temperature⁃responsive copolymers can be linked to the surfaces of glass spheres by bottom⁃spray coating technology, and the copolymer layers with certain thickness can be formed on the sphere surfaces. The composite carriers have good biocompatibility, and can realize effective cell adhesion and spontaneous desorption by using its intelligent response characteristics.

The original anthocyanins from peanut skin can be extracted by using ultrasonic assisted solvent extraction, and the extraction conditions of anthocyanins can be optimized by response surface methodology. Based on the single factor test analysis and Box-Behnken experimental design principle, the optimal conditions which is suitable for the production of the peanut red skin proanthocyanidins was chosen according to the response surface methodology as follows: ethanol concentration is 70%, the solid-liquid ratio is 1∶50, ultrasonic power is 153 W, ultrasonic time is 8 min, the boiled temperature is 60 ℃, the boiled time is 50 min and extraction once. Under this optimum conditions, proanthocyanidins yield is 153.63 mg/g and is very close to the mode expected value 154.33 mg/g.

Temperatureresponsive culture substrates containing poly(Nisopropylacrylamide) (PNIPAAm) have been developed as an effective substitute for enzymatic treatment to recover adherent cells, which can control cell attachment and detachment by changing temperature. However, not all PNIPAAmcontaining surfaces can be applicable to cell culture and recovery. That means adherent cells show accommodative selectivity for thermoresponsive substrates. In this study, different cells including HeLa cells, HEK293 cells, bone marrow mesenchymal stem cells (BMMSCs) and adiposederived stem cells (ADSCs) were cultured on the temperatureresponsive PNIPAAm copolymer films with different surface wettability, topography and film thickness. The experimental results demonstrate that various cells had their special thermoresponsive substrates, and the optimal copolymer films were selected for HeLa cells, HEK293 cells, BMMSCs and ADSCs respectively.

Using poly(3hydroxybutyrateco3hydroxyvalerate) (PHBV) as sole carbon source, the biodegradation of the PHBV by [WTBX]Pseudomonas mendocina[WTBZ] DS04T was studied. The effect of incubation time, cultivation temperature, initial pH, shaking revolution, liquid medium volume and inoculation content on PHBV degradability were studied. According to the results of orthogonal test, the optimal conditions of enzyme production were chosen as follows: temperature 28 ℃, incubation time 32 h, initial pH 7.5, shaking revolution 150 r/min, inoculation content 1.5% , and 100 mL culture medium is load in 250 mL flask. Under the optimum conditions, the PHBVdegrading enzymatic activity reached (18.9±1.2) U/mL.